Francois of France (September 12, 1494-Febuary 24, 1525) m. Claude of Brittany (October 13, 1499-July 20, 1524).
1. Princess Louise (August 19 1515-September 18, 1518).
2. King Charles IX (b. October 23 1516). m. Mary of England (February 18 1516).
1. Dauphin Henri (b. November 7, 1533)
2. Prince Francois (b. June 8, 1536).
3. Prince Francois, Duke of Orleans. (b. February 28 1518). m. Maria of Saxony (b. 1515).
4. Prince Henri, Duke of Angoulême (b. March 31 1519). m. Catherine de' Medici (b. 1519).
5. Princess Madeline (b. August 10, 1520). m. King James of Scots (April 10 1512).
6. Prince Louis, Duke of Berry (b. January 22, 1522).
7. Princess Marguerite (b. June 5, 1523) m. Infante Antonio (b. June 13 1522).
Philip the Handsome (July 22 1478-September 25, 1506)m. Juana of Aragon (b, November 6 1479
1. Eleanor of Austria (b. November 15 1498) m. King John III of Portugal (June 7 1502).
1. Infante Antonio (b. June 13 1522). m. Princess Marguerite (b. June 5, 1523).
2. Infanta Maria (b. October 15 1523).
3. Infante Alfonso (b. February 24 1526-1527).
4. Infanta Isabel (b. June 18 1527-1531).
5. Infanta Juana (b. February 15 1530).
6. Infante Manuel (b. November 18 1531-1537).
7. Infante Densis (April 5 1533).
2. Holy Roman Emperor Charles V (b. February 24 1500) m. Isabel of Portugal (b. October 24 1503).
1. Infanta Margaret (b. July 5, 1522).
2. Infante Philip (b. March 19, 1525).
3. Infante Ferdinand (1527-1531).
4. Infanta Maria (b. June 21, 1528).
5. Infante Carlos (b. November 22, 1529).
6. Infante Juan (b. June 29, 1534).
7. Infanta Juana (b. June 24, 1535).
3. Archduchess Isabella (July 18 1501 – January 19 1526) m. Christian II of Denmark (b. July 1, 1481).
1. Prince John (b. February 21 1518-1532).
2. Princess Dorothea (b. November 10, 1520). m. Frederick II, Elector Palatine (b. December 9, 1482).
3. Princess Christina (b. 1522). m. Francesco II Sforza, Duke of Milian (February 4, 1495 – November 2, 1535)
4. Archduke Ferdinand (b. March 10 1503) m. Princess Anna of Hungary (b. July 23 1503).
1. Archduchess Elizabeth (b. July 9 1526).
2. Archduke Maximilian (b. July 27 1527).
3. Archduchess Anna (b. July 7, 1528).
4. Archduke Ferdinand (b. June 14, 1529).
5. Archduchess Maria of Austria (b. May 1531).
6. Archduke Magdalena of Austria (b. August 14, 1532).
7. Archduchess Catherine of Austria (b. September 15, 1533).
5. Archduchess Mary (b. September 15, 1505) m. Louis II of Hungary (b. July 1 1506).
1. Prince Károly (b. August 29 1523).
6. Archduchess Catherine (b. January 14, 1507) m. Prince Henry of Wales (b. January 1 1511).
1. Princess Catherine (b. April 28, 1529).
2. Princess Elizabeth (b and died 1530).
3. Prince Edward (November 1 1531-July 30 1537).
4. Princess Elizabeth (March 15 1533).