ATL familiy of HRE Charles V, ancestor of Spanish Habsburgs, English Habsburgs and Scottish Habsburgs:
Charles V (1500-1558) Holy Roman Emperor, King of Spain, m. Isabella of Portugal (1503-1539)
1) Maria (1527-1598) m. Maximilian II (1527-1576) Holy Roman Emperor
1) Anna (1549-1601) m. Edward II (1540-1596) King of Portugal [1]
2) Ferdinand II (1550-1606) Holy Roman Emperor, m. Maria Anna of Bavaria (1551-1608)
3) Elizabeth (1552-1589) m. Sigismund III Casimir (1545-1602) Grand Duke of Lithuania, King of Poland [2]
4) Rudolf (1553)
5) Mary (1555-1601) m. Henry IX of England (1555-1605)
6) Eleanor (1556-1618) m. Charles IX (1550-1574) King of France
7) Frederick (1558)
8) Ernest (1561-1610) Archduke of Tyrol, m. Anna of Austria-Tyrol (1585-1618)
9) Albert (1564-1567)
10) Matthias (1566-1607) Grand Master of Teutonic Order
2) Philip (1528-1603) King of Spain, m. a) Maria Manuela of Portugal (1527-1557), b) Maria of Portugal (1538-1577) [1]
1a) Isabella (1547)
2a) Charles (1550-1556)
3a) Catherine (1552)
4b) Charles (1560-1565)
5b) Isabella (1561-1562)
5b) Philip III (1564-1606) King of Spain, m. Anna of Portugal (1570-1633) [3]
6b) Joanna (1566-1598) m. Manuel II (1568-1615) King of Portugal [3]
7b) Maria (1567-1570)
8b) Catherine (1569-1630) m. James VI (1568-1616) King of Scotland
9b) Ferdinand (1572-1591)
3) Joanna (1529-1530)
4) Isabella (1534)
5) Ferdinand (1535-1573) Duke of Burgundy, m. a) Mary (1516-1558) Queen of England, b) Mary (1542-1594) Queen of Scots
1a) Henry IX (1555-1605) King of England, Duke of Burgundy, m. Mary of Austria (1555-1601)
2b) Mary (1565-1571)
3b) James VI (1568-1616) King of Scotland, m. Catherine of Spain (1569-1630)
4b) Charles (1570)
5b) Mary (1573-1615) m. Charles X (1574-1638) King of France [4]
6) Isabella (1537-1538)
[1] Children of Duarte, Duke of Guimarães, younger brother of John III. As John III dies without surviving issue he is succeeded by nephew.
[2] Son of Sigismund Augustus and Elizabeth of Austria
[3] Children of Edward II of Portugal and Anna of Austria.
[4] Posthumous son of Charles IX