Sure!
Margaret,
Archduchess of Austria (b.1480: d.1530) m. Charles VIII,
King of France (b.1470: d.1498) (a) -annulled 1491-, Juan,
Prince of Austrias (b.1478: d.1497) (b)
1b) Isabella II, Queen of Castile and Aragon (b.1498: d.1534) m. Charles V, Holy Roman Emperor (b.1500: d.1558) (a)
1a) Philip, Prince of Asturias (b.1520: d.1528)
2a) Ferdinand III, King of Spain (b.1522: d.1549) m. Maria, Infanta of Portugal (b.1521: d.1546) (a), Maria Manuela, Infanta of Portugal (b.1527: d.1578) (b) [1]
1a) Margaret, Infanta of Spain (b.1542)
2a) Catherine, Infanta of Spain (b.1543)
3a) Stillborn Son (c.1545)
4a) Miscarriage (c.1546)
5b) Philip II, King of Spain (b.1548)
6b) Stillborn Daughter (c.1549)
7b) Isabella, Infanta of Spain (b.1550: d.1550)
3a) Margaret, Archduchess of Austria (b.1523: d.1523)
4a) Stillborn Son (c.1525) - twin
5a) Stillborn Son (c.1525) - twin
6a) Maximilian II, Holy Roman Emperor (b.1527: d.1584) m. Marguerite, Princess of France (b.1523: d.1574) (a)
1a) Philip, Archduke of Austria (b.1549: d.1550)
2a) Elisabeth, Archduchess of Austria (b.1552)
3a) Stillborn Son (c.1554)
4a) Maximilian, Archduke of Austria (b.1555: d.1558)
5a) Joanna, Archduchess of Austria (b.1557: d.1557)
6a) Marie, Archduchess of Austria (b.1559: d.1569)
7a) Alexandre I, Holy Roman Emperor (b.1562)
8a) Stillborn Daughter (c.1563)
7a) Joanna, Archduchess of Austria (b.1530: d.1532)
8a) Stillborn Daughter (c.1530)
9a) John, Archduke of Austria, Viceroy of Naples (b.1533: d.1580) m. Mary I, Queen of Scotland (b.1542: d.1595) (a)
1a) John II, King of Scotland (b.1563)
2a) Miscarriage (c.1564)
3a) Stillborn Daughter (c.1565)
4a) Margaret, Princess of Scotland (b.1566)
5a) James, Duke of Albany (b.1569: d.1581)
6a) Maximilian, Duke of Ross (b.1576)
7a) Stillborn Daughter (c.1578)