Juan III, King of Castile and Aragon (1478 - 1519) married Margaret of Austria (1480 - 1542) in 1497
- Isabel (b. 1497) married Miguel, King of Portugal (b. 1498) in 1514.
- Fernando VI, King of Castile, Aragon and Navarre (b. 1499) married Madeleine, Queen of Navarre (b. 1494) in 1513.
- Juana (b. 1500) married Henry VIII, King of England (b. 1491)
- Maria (b. 1502) married Louis XIII, King of France (b. 1500) in 1518. [1]
- Catalina (1504 - 1511)
- Juan, King of Sicily and Naples (b. 1507) married
Eleanor d'Este (b. 1515) in 1531 Isabella Maria d'Este (b. 1519) in 1533. [4]
- Alfonso, Archbishop of Toledo (b. 1509)
- Leanor (b. 1510) married
Charles V, Holy Roman Emperor (b. 1500) in 1526 as second wife Philibert III Duke of Savoy (b. 1504) in 1526. [2]
- Margarita (b. 1512) married Maximilian II Sforza, Duke of Milan (b. 1513) in 1528 [3]
- Beatriz (b. 1514) married Francis I, Duke of Lorraine (b. 1517) in 1533.
[1] Son of Anne of Britanny and Louis XII.
[2] Son of Philibert II and Yolande Louise of Savoy.
[3] Son of Maximilian I Sforza and Bona Sforza.
[4] OTL she was died as an infant. ATL she lives.