Philip II of Spain (b.1527: d.1598) m. Maria Manuela of Portugal (b.1527: d.1545) (a), Mary I of England (b.1516: d.1558) (b), Elizabeth I of England (b.1533: d.1603) (c)
1a) Carlos, Prince of Wales (b.1545: d.1568) m. Elisabeth de Valois (b.1545) (a)
1a) Philip III of Spain (b.1565: d.1610) m. Margaret of Austria (b.1567: d.1633) (a)
1a) Charles II of Spain (b.1584: d.1611)
2a) Philip IV of Spain (b.1586)
3a) Maria Isabella of Spain (b.1589)
4a) Stillborn Boy (c.1591)
5a) Juana of Spain (b.1594)
6a) Ferdinand of Spain (b.1598)
2a) Ferdinand of Spain (b.1567: d.1568)
3a) Stillborn Girl (c.1569)
2c) Henry, Prince of Wales (b.1560: d.1589) m. Catherine de Bourbon (b.1559: d.1604) (a)
1a) Philip I of England (b.1582)
2a) Edward, Duke of York (b.1585)
3a) Charles, Duke of Clarence (b.1587)
3c) Margaret of Spain (b.1562: d.1619) m. Henry II, Duke of Lorraine (b.1563: d.1624) (a)
1a) John de Lorraine (b.1585: d.1588)
2a) Philippe de Lorraine (b.1586: d.1588)
3a) Louis de Lorraine (b.1587: d.1588)
4a) Marie Claude de Lorraine (b.1590)
5a) Stillborn Boy (c.1591)
6a) Henri III, Duke of Lorraine (b.1593)
7a) Stillborn Boy (c.1594)
8a) Francis de Lorraine (b.1595: d.1599)
9a) Stillborn Boy (c.1596)
10a) Christine de Lorraine (b.1598)
11a) Nicole de Lorraine (b.1598)
4c) Isabella of Spain (b.1565) m. James VI of Scotland (b.1566: d.1625) (a)
1a) James, Duke of Rothesay (b.1584: d.1602)
2a) Charles I of Scotland (b.1589)
3a) Elizabeth Stuart (b.1594)
5c) Edward, Duke of Burgundy (b.1566: d.1601) m. Marie Elisabeth of France (b.1572: d.1593) (a), Elizabeth of Denmark (b.1573: d.1626) (b)
1a) Philip, Duke of Burgundy (b.1590)
2a) Elisabeth of Burgundy (b.1592: d.1593)
3b) John of Burgundy (b.1595: d.1599)
4b) Catherine of Burgundy (b.1596)
5b) Charles of Burgundy (b.1599)
6b) Margaret of Burgundy (b.1602)