| Office | Office Holder | Political Party | Home State | Preceded by | Followed by |
| Executive Chair | Thomas Jefferson | Democratic-Republican | Virginia | George Washington | Alexander Hamilton |
| Constitutional Chair | Alexander Hamilton | Federalist | New York | George Mason | Thomas Jefferson |
| Chair of State | John Adams | Federalist | Massachusetts | Thomas Jefferson | James Madison |
| Chair of the Treasury | James Madison | Democratic-Republican | Virginia | Alexander Hamilton | John Adams |
| Chair of Justice | John Marshall | Federalist | New York | John Jay | John Marshall |
| Chair of Defense | John Paul Jones (through 1799) | American | New York | Henry Knox | Office Ended |
| Chair of the Interior | James Monroe | Democratic-Republican | Virginia | John Adams | James Monroe |
| Chair of the Army | John Paul Jones (from 1799) | American | New York | Office Created | John Quincy Adams |
| Chair of the Navy | John Paul Jones (from 1799) | American | New York | Office Created | William Bainbridge |