Mary I, Queen of england (1516-1565),mJames V, King of Scotland and I, King of England (1512-1562)
James VI of Scotland and II of England (1533-1590) mJohanna of Austria (1535-1589) with issues
Catherine (1535-1580) m John Manuel, Prince of Portugal (1537-1554) with issues
Mary (1537-1580) m John of Austria, Duke of Burgundy (1537-1576) with issues
miscarriage
Charles, Duke of York and Albany (1540-1620) mDorothea of Lorraine (1544-1612) with issues
miscarriage
Margaret (1543-1623) mFrancis II of France (1544-1563) with
miscarriage