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As it says on the tin, Carlos, Prince of Viana (only son of Juan II of Aragon and his Navarrese first wife), has issue. This can be him having kids by Agnes of Cleves (which will be interesting, if only for their familial connections – most specifically in France and Burgundy). Or successfully remarrying after Agnes’ death – he wanted to marry the Dowager Duchess of Brittany, Isabella of Scots or Infanta Catarina of Portugal (sister of Holy Roman Empress Leonor). Either way, somehow – he leaves surviving issue (and maybe even survives his dad). Where do things go from here?
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