Not really. The Emperor had a lot of trouble and rember who Henry was still an anointed King and Mary his subject... The Emperor had too many enemies at that time for fighting also England over the question of Anne, Mary and Catherine and if he (like OTL already shortly before Anne's fall) need the alliance of England against France (and still more important need who England do not ally with France against him) and Henry had a male son by Anne, you can be sure who Karl V can be persuaded to recognize the validity of Henry and Anne marriage, recognize Anne as legitimate Queen of England and her son as Prince of Wales in exchange of an alliance and the support of English ship and soldiers against France
I was actually talking about the (admittedly remote) chances of Henry beheading Mary if she doesn't accept being made illegitimate, but fair enough.
Oh, and I forgot, Catherine of Aragon is still alive when *Edward was born, how do you think she'll react?