When the 13 colonies of the United States rebelled against the mother country and formed a union which would become the United States they diffed quite a bit culturally. The puritans of the New England colonies differed greatly from the Dutch culture of New York, the Quaker and German culture of Pennsylvania, the Cavalier culture of Virginia, and the West Indies culture of South Carolina and Georgia to say nothing of the Scots Irish in Appalachia. Books like American Nations by Colin Woodard, and David Hackett Fishers' Albion's Seed theorize that the cultural divisions laid out by these colonies still persist strongly to this day. However necessity brought them together as any of the colonies going it alone would be easy prey for colonial powers. So my question is what if anything could keep these colonies from unifying and in what scenario could they survive and with their manifest destiny result in a timeline where there's multiple nation states on the North American continent?