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Viceroy of Valencia Johann Hohenzollern from Franconian line of House of Hohenzollern, second husband of Germaine de Foix, step-grandmother of Emperor Charles V died childless in 1525 aged 32. What if Johann lives longer (say he outlives Germaine and dies in 1545) and have surviving sons with Germaine (say they have 3 sons-Juan, Carlos, Federico. Born in 1521, 1523, 1527)? So Johann would remain Viceroy untill his death, but what about his sons? Would they just merge with Spanish higher aristocracy? Initially it would not have significant impact on history, but assuming, that descendants of Johann's older brothers would be extinct in male line like IOTL (last of them, Georg Friedrich of Ansbach died in 1598) these Valencian Hohenzollerns would inherit Franconia, and later-Ducal Prussia, as closest male-line relatives of Duke Albrecht Friedrich.
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