POD: Charles V dies before 1525. Redo of this
post.
Holy Roman Emperor Ferdinand I (IV of Spain) (1503- 1554) m. Empress Anna of Hungary (1503-1547).
1. Archduchess Elizabeth of Austria (1526-1545) m. Sigismund II Augustus of Poland (1520-1572).
2. Holy Roman Emperor Maximilian II (1527-1576) m. Margaret of Valois (1523-1574).
1. Anna of Austria, Queen of Portugal (1547-1580) m. King Carlos I of Portugal (1545-1588).
1. Infante Ferdinand (1571-1578).
2. Infante Carlos (1571-1573)
3. King Diego I of Spain and Portugal (1575-1622).
4. Infante Felipe (1578-1621).
5. Infanta Maria (1580-1583).
2. Archduke Ferdinand (1549-1552)
3. Holy Roman Emperor Francis I (1550-1612).
4. Archduke Rudolf (1553-1595)
5. Elizabeth of Austria (1554-1592) m. Henri Emmunel, Duke of Savoy (1552-1620).
6. Margaret of Austria (b and d 1555).
7. Matthias of Austria (1557-1619).
8. Maximilian of Austria (1560-1618).
9. Charles of Austria (1562-1631).
10. Margaret of Austria (1564-1633).
3. Archduchess Anna of Austria (1528-1590) m. Albert IV, Duke of Bavaria (1528-1579)
4. King Carlos II of Spain (1529-1595). m. Maria Manuela of Portugal (1528-1545). (a) Elizabeth of France (1543-1590) (b).
1a. Carlos I of Portugal (1545-1588). m. Archduchess Anna of Austria (1547-1580).
2b. Infanta Anna Clara (1564-1633).
3b. Infanta Maria Michaela (1566-1604).
4b. Infanta Isabella Eugenia (1567-1597).
5b. Infanta Juana (b and d 1560).
6b. Infanta Margarita Catalina (1565-1617).
5. Archduchess Maria of Austria (1531-1581). m. William, Duke of Cleves (1516-1592).
6. Archduchess Magdalena of Austria (1532-1590).
7. Archduchess Catherine of Austria (1533-1572). m. Fredrick III, Elector of the Palatine [1525-1588].
8. Archduchess Eleanor of Austria (1534-1594) m. King Edward VI of England (1531-1563)
9. Archduchess Margaret of Austria (1536-1567).
10. Archduke John (1538-1539).
11. Archduchess Barbara of Austria (1539-1572) m. João Manuel, Prince of Portugal (1537-1544) (a) m. Guglielmo Gonzaga, Duke of Mantua [1538-1587) (b)
12. King Ferdinand II of Hungary (1540-1590) m. Renata of Lorraine (1544-1602).
13. Archduchess Ursula of Austria (1541-1543)
14. Archduchess Helena of Austria (1543-1574).
15. Archduchess Joanna of Austria (1547-1608). m. Alfonso II d'Este, Duke of Ferrara (1533-1597).