In January 1582, Alexander IV authorised the burning at the stake of Protestants for heresy, Protestant services had been banned since July 1569, Protestant lords gave shelter to their co-religionists and places to worship in their houses.
Catherine, Queen of Scots, at first supported her husband's religious policy. But after hearing reports of the bravery of people dying in agony for their faith, she changed her mind. She pleaded many times with her husband to end the persecution. But he refused, saying that he was doing God's work in stopping the spread of heresy, and those sentenced to death were given every opportunity to recant. On 8 May 1587, she met Archibald Campbell, Earl of Argyll, and seven other Protestant lords, at his town house in Edinburgh. They decided that she would ask her husband to end the persecution of fellow Scots, If he refused, they would demand his abdication.
Catherine met Alexander in the garden of Holyrood Palace the next day. He refused her request to end religious persecution. Then the Protestant lords appeared, They were armed, They demanded that he abdicate. He refused and called the guards. There was a fight between them and the lords. One of the guards stabbed and killed Campbell. The other lords then stabbed the king to death. Afterwards they proclaimed his and Catherine's four year son aa James VII, King of Scots,
Catherine became Regent for her son. The following day she ended the persecution of Protestants, So no more were burnt at the stake for heresy, and they could now worship in their churches. 233 Protestant men and women died for their faith in five years.