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Fate of the two daughters
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Mary Tudor, Queen of Scots and Spain

In 1528 Wolsey's agent Thomas Magnus discussed an idea of Mary Tudor’s marriage to James V of Scotland with the Scottish diplomat Adam Otterburn and this would come to pass in 1532, when Mary Tudor was sixteen, this was Mary Tudor’s first marriage which would be with James V of Scotland, effectively ending the Auld alliance and she would have two surviving daughters, Catherine Stewart (December 16, 1536) and Mary Stewart (December 8, 1542), On 1543, James V would die and leave Mary Tudor a widow and betrothed to Charles V once again as she was expelled from the regency by the prospective husband of Catherine Stewart, Mathew Stewart, duke of Lennox, however the death of Maria Manuela and her child due to a miscarriage in 1544 would mean that Mary Tudor would marry once again to Philip II of Spain but this time she would bear two children, Infante Carlos(June 2, 1548) and Infanta Juana(December 20, 1550), she would die in 1551 of natural causes.

On the spring of 1535, Queen Anne of Bohemia and Hungary would suffer miscarriage a and Ferdinand, Archduke of Austria needed another wife and he would marry Katherine Tudor, the second daughter of Katherine of Aragon and Henry VIII, she is seventeen at the time of the marriage and would have been crowned as the second queen of Bohemia and Hungary as well of Austria, she would give birth to the following children who would be carried into term and survived infancy; Margaret of Austria(February 16, 1536), Barbara of Austria(April 10, 1538), Charles of Austria(June 3, 1540) and Joanna of Austria(January 24, 1543), she would be crowned as Holy Roman Empress consort with her husband Ferdinand I on the abdication of Charles V.

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